AmpliScribe™ T7-Flash™ Transcription Kit/AmpliScribe™ T7快速体外转录试剂盒
货号 | ASF3507/ASF3257 | 售价(元) | 4253/2831 |
规格 | 50T/25T | CAS号 |
- 产品简介
- 相关产品
产品信息
货号 |
产品名称 |
规格 |
价格/元 |
ASF3507/ASF3257 |
AmpliScribe™ T7-Flash™ Transcription Kit/AmpliScribe™ T7快速体外转录试剂盒 |
50T/25T |
4253/2831 |
AS3107 |
AmpliScribe™ T7 High Yield Transcription Kit,AmpliScribe™ T7高产转录试剂盒 |
50T |
3191 |
DS010910/DS010925 |
DuraScribe T7 Transcription Kit/核酸酶耐受体外转录试剂盒 |
10T/25T |
4567/8466 |
ASB71110 |
AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit 生物素RNA体外转录试剂盒 |
10T |
5501 |
AS3107 |
AmpliScribe T7 High Yield Transcription Kit/高产体外转录试剂盒 |
50T |
3931 |
AmpliScribe™T7-Flash™转录试剂盒经过特殊配制,使用户能够在30分钟内从体外转录反应中获得最高产量的RNA。 标准的30分钟,20μLAmpliScribe T7-Flash反应将从1μg线性对照DNA模板中产生160-180μgRNA。
AmpliScribe T7-Flash转录试剂盒可用于产生各种大小的RNA转录本(从<50 b到> 9 kb)。 标准的AmpliScribe T7-Flash反应也可以按比例放大,以在单个反应中产生毫克量的RNA。
优点:
通过30分钟的高产量方案节省时间
扩大反应以产生克级别RNA
组成成分:
T7 RNA Polymerase:120μL;Store only at –20°
10X Transcription Reaction Buffer:250μL;Store only at –20°
ATP (100 mM):100μL;Store only at –20°
GTP (100 mM):100μL;Store only at –20°
CTP (100 mM):100μL;Store only at –20°
UTP (100 mM):100μL;Store only at –20°
DTT (100 mM):125μL;Store only at –20°
RiboGuard RNase Inhibitor (40 U/µL):30μL;Store only at –20°
Nuclease-Free Water, Sterile:1.0mL;Store only at –20°
Control Template DNA (0.5 µg/µL) :25μL;Store only at –20°
RNase-Free DNase I (1 U/µL):60μL;Store only at –20°
参考文献:
1. Molecular Cloning - A Laboratory Manual, Third Edition, 2001. CSHL Press. pp 7.27 – 7.34. J. Sambrook and D. Russell.