产品信息

      AmpliScribe™T7高产转录试剂盒特殊配方使得用户能够从体外转录反应中获得最大可能的RNA产量。利用1μg对照模板在标准的2小时，20-μL AmpliScribe反应体系中，将掺入反应体系中高达90％的NTP，并产生高达150μg的RNA。

      The AmpliScribe™ T7 High Yield Transcription Kit is specially formulated to enable users to obtain the maximum possible yields of RNA from an in vitro transcription reaction. The standard 2-hour, 20-µL AmpliScribe reaction will incorporate up to 90% of input NTPs and produce up to 150 µg of RNA from 1 µg of the control template. These yields are made possible by the high-performance properties of the AmpliScribe T7 enzyme preparations.

      AmpliScribe Kits produce exceptionally high yields of either long or short transcripts. The standard AmpliScribe reaction can be scaled up to produce milligram amounts of RNA. In addition, an AmpliScribe reaction can be readily modified to prepare fluorescent-¹, biotinylated-², or digoxigenin-³labeled RNA. Produce high yields of high quality RNA quickly and easily.

Formerly from Epicentre Synthesize long and short RNA with one kit

Produce >20 times more RNA than conventional or "homemade" in vitro transcription kits

Components:

AmpliScribe T7 Enzyme Solution, 

100 mM ATP, CTP, GTP, and UTP Solutions, 

AmpliScribe 10X Reaction Buffer, 

100 mM Dithiothreitol (DTT), 

RiboGuard™ RNase Inhibitor, 

RNase-Free Water, 

Control Template DNA, and RNase-Free DNase I

参考文献：

DeLong, E.F. et al., (1999) Appl. and Environ. Microbiol., 56, 5554.

Hoffman, L.M. and Johnson, M.G. (1994) BioTechniques 17, 372.

Kaplan, E.D. et al., (1996) Epicentre Forum, 3 (2), 1.

Molecular Cloning - A Laboratory Manual, Third Edition, 2001. CSHL Press. pp 7.27 –7.34. J. Sambrook and D. Russell.