HighYield T7 Atto488 RNA Labeling Kit
| 货号 | RNT-101-488-S | 售价（元） | 5925 |
| 规格 | 20x 20 μl | CAS号 |
- 产品简介
- 相关产品
产品信息
|
品牌 |
货号 |
名称 |
规格 |
单价 |
|
Jena Bioscience |
20Tx 20 μl |
5925.00 |
HighYield T7 Atto488 RNA标记试剂盒设计用于通过体外转录随机产生Atto488RNA修饰探针。这种探针非常适合原位杂交和Northern Blot实验。UTP-ATTO-488使用优化的反应缓冲液和T7 RNA标记聚合酶混合物有效地并入RNA中，作为其天然对应物UTP的替代物。与荧光素相比，ATTO488具有极好的光稳定性。35%UTP-ATTO-488替代通常会在反应和标记效率之间实现最佳平衡。然而，UTP-ATTO-488/UTP比值的单独优化可以通过单核苷酸格式轻松实现。由此产生的Atto488修饰RNA探针随后可以通过荧光光谱检测。
该试剂盒含有足够的试剂，用于20个标记反应，每个反应20μl（35%UTP-ATTO-488替代物、2.5 mM ATP、GTP、CTP、0.2 mM UTP、0.1 mM UTP-ATTO-488）。
Content:
HighYield T7 RNA Labeling Polymerase Mix
RNT-101-488-S: 2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)
RNT-101-488-L: 3x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)
HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based
ATP - Solution
1x 100 μl (100 mM)
GTP - Solution
1x 100 μl (100 mM)
CTP - Solution
1x 100 μl (100 mM)
UTP - Solution
1x 100 μl (100 mM)
UTP-ATTO-488
RNT-101-488-S: 2x 20 μl (1 mM)
RNT-101-488-L: 5x 20 μl (1 mM)
T7 G-initiating control template (1.4
kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor
resulting in approx. 1400 nt RNA transcript
PCR-grade water
1x 1.2 ml
DTT
1x 100 μl (100 mM)
To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)
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RNT-101-AF488 |
HighYield T7 AF488 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
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RNT-101-488-S |
HighYield T7 Atto488 RNA Labeling Kit |
20reactions x 20 μl |
5925.00 |
|
Jena Bioscience |
RNT-101-488-L |
HighYield T7 Atto488 RNA Labeling Kit |
50reactions x 20 μl |
13755.00 |
除上述mRNA体外合成试剂盒外，Jena Bioscienc还提供一系列相关高质量原料。包括：修饰核苷酸、T7 RNA聚合酶、加帽酶、加尾酶等。
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