HighYield T7 RNA Synthesis Kit
| 货号 | RNT-101 | 售价（元） | 2925 |
| 规格 | 50T x 20 μl | CAS号 |
- 产品简介
- 相关产品
产品信息
|
品牌 |
货号 |
名称 |
规格 |
单价 |
|
Jena Bioscience |
50T x 20 μl |
2925.00 |
产品简介
HighYield T7 RNA合成试剂盒旨在通过T7 RNA聚合酶的体外转录产生大量RNA。它也适用于加入修饰核苷酸（例如生物素或荧光标记的NTP或cap类似物）。
由此产生的RNA随后可用于多种应用，如微注射、转染或体外翻译实验、结构/功能研究或原位杂交探针和Northern Blot实验。
该试剂盒包含足够的试剂，用于50个反应，每个反应20μl（每个NTP 7.5 mM）。培养30分钟后，20μl反应产生约140-160μg RNA（1μg T7对照模板，1.4 kb RNA转录物）。然而，产量可能因模板而异（启动子设计、序列长度、二级结构形成）。
Content:
HighYield T7 RNA Polymerase Mix
3x 40 μl incl. RNase inhibitor and 50 % glycerol (v/v)
HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based
ATP - Solution
1x 100 μl (100 mM)
GTP - Solution
1x 100 μl (100 mM)
CTP - Solution
1x 100 μl (100 mM)
UTP - Solution
1x 100 μl (100 mM)
T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor
resulting in ~1400 nt RNA transcript
T7 A-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class II phi2.5 promotor
(A-initiating) resulting in ~1400 nt RNA transcript
PCR-grade water
1x 1.2 ml
DTT
1x 100 μl (100 mM)
To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I
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20reactions x 20 μl |
4875.00 |
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20reactions x 20 μl |
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|
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50reactions x 20 μl |
13755.00 |
除上述mRNA体外合成试剂盒外，Jena Bioscienc还提供一系列相关高质量原料。包括：修饰核苷酸、T7 RNA聚合酶、加帽酶、加尾酶等。
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