2-Deoxy-D-glucose (2-DG)
| 货号 | ICE1201 | 售价（元） | 567 |
| 规格 | 1g | CAS号 | 154-17-6 |
- 产品简介
- 相关产品
产品信息
|
货号 |
产品名称 |
规格 |
价格/元 |
|
ICE1201-0001G |
2-Deoxy-D-glucose 2-脱氧-D-葡萄糖 |
1g |
567 |
|
ICE1201-0005G |
2-Deoxy-D-glucose 2-脱氧-D-葡萄糖 |
5g |
2298 |
|
ICE1201-0025G |
2-Deoxy-D-glucose 2-脱氧-D-葡萄糖 |
25g |
询价 |
2-脱氧-D-葡萄糖（2-Deoxy-D-glucose, 2-DG），一种不可代谢的葡萄糖类似物，通过作用于己糖激酶（糖酵解的限速步骤）来抑制糖酵解。2-DG被己糖激酶磷酸化生成2-DG-P，后者不能被磷酸葡萄糖异构酶进一步代谢，进而引起2-DG-P在细胞内的累积和细胞内ATP的耗尽。体外缺氧模型中，2-DG导致细胞周期抑制和细胞死亡，诱导自噬，增加ROS生成和活化AMPK。体内动物模型中阻断肿瘤细胞生长。
产品特性
1） CAS NO：154-17-6
2） 化学名：(4R,5S,6R)-6-(Hydroxymethyl)oxane-2,4,5-triol
3） 同义名：2-deoxyglucose; 2-Deoxy-D-arabinohexose; D-Arabino-2-deoxyhexose; Ba 2758; NSC 15193; 2-脱氧葡萄糖；2-去氧-D-葡萄糖；D-2-脱氧葡萄糖；
4） 分子式：C6H12O5
5） 分子量：164.16
6） 外观：白色至类白色固体
7） 纯度：>98%
8） 溶解性：溶于DMSO（20mg/ml）、H2O（30mg/ml）、PBS, pH 7.2 （10mg/ml）
保存与运输方法
保存：2-8℃避光干燥保存，至少2年有效。
运输：室温运输。
注意事项
1） 本品储存液置于≤-20℃避光保存，尽量避免反复冻融。通常情况下，-20℃至少一个月稳定，-80℃至少6个月稳定。
2） 本品仅用作科研用途，绝不可用于临床诊断或治疗药物，绝不可用做食品或药品。
3)为了您的安全和健康，请穿实验服并戴一次性手套操作。
储存液配制
|
质量 浓度 |
1mg |
5mg |
10mg |
|
1mM |
6.0916 mL |
30.4581 mL |
60.9162mL |
|
5mM |
1.2183mL |
6.0916 mL |
12.1832mL |
|
10mM |
0.6092 mL |
3.0458 mL |
6.0916 mL |
|
50mM |
0.1218 mL |
0.6092 mL |
1.2183 mL |
使用方法【源自文献，仅作参考】
文献1，Yan J et al. The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells. Cancer Res. 2005 Aug 1;65(15):7023-30. PMID: 16061689
体外研究（细胞实验）：
细胞类型（Cell types）：MCF-7 cells
实验方法（Assay）：For cell growth assay, MCF-7 cells were plated at 3 × 104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium. After 24 hours, cells were fed with fresh medium including 2-DG at doses of 0, 4, 8, or 16 mmol/L. At days 1, 3, and 5 after 2-DG exposure, cells were fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates were stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance was determined at 590 nm.
实验结果（Result）：Cell growth was significantly inhibited by 2-DG at doses of 4, 8, or 16 mmol/L for 1, 3, or 5 days in a dose- and time-dependent manner (P < 0.001).
体内研究（动物模型）：
动物模型（Animal Model）：At 21 days of age, rats were injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Six days following carcinogen injection, all rats were randomized into one of three groups, 30 rats per group, and werefed ad libitum AIN-93G diet containing0.0%, 0.02%, or 0.03% (w/w) 2-DGfor 5 weeks.
实验结果（Result）：2-DG decreased the incidence and multiplicity of mammary carcinomas and prolonged cancer latency (P < 0.05). The 0.02% dose of 2-DG had no effect on circulating levels of glucose, insulin, insulin-like growth factor-I, IGF binding protein-3, leptin, or body weight gain.
文献2，Wang H et al. 2-deoxy-D-glucose enhances anesthetic effects in mice. Anesth Analg. 2015 Feb; 120(2):312-9. PMID: 25390277
体内研究（动物模型）：
动物模型（Animal Model）：Female Sprague-Dawley rats at 20 days of age
配制方法（Formulation）：2-DGwasdiluted insaline.
实验方法（Assay）：2-DG (1000 mg/kg), 3-NP (50 mg/kg),30 or saline was injected [intraperitoneally (ip)] 5 minutes before isoflurane anesthesia.Idebenone (200 mg/kg) or L-carnitine (100 mg/kg) was injected 15 minutes before isoflurane anesthesia (10 minutes before treatment with 2-DG or 3-NP).
实验结果（Result）：2-DG enhances isoflurane-induced loss of righting reflex (LORR) in mice.