Product Information：

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.

Description：

Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.

Applications：IP, CoIP, ChIP, RIP

Specificity/Target：AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP.For the complete list, please click here: Fluorescent protein specificity table

Binding capacity：25-30 µg of recombinant GFP per 25 µL bead slurry

Conjugate：Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)

Elution buffer：

SDS sample buffer

0.2 M glycine pH 2.5

Wash buffer compatibility：

1 mM DTT,

3 M Guanidinium•HCl, 8 M Urea,

2 M NaCl,

2 % Nonidet P40 Substitute,

1 % SDS,

1 % Triton X-100

Type：Nanobody

Class：Recombinant

Host：Alpaca

Affinity (KD)：Dissociation constant KD of 1 pM

Compatibility with mass spectrometry：The GFP-Trap® is optimized for on-bead digestion. On-bead digest protocol for mass spectrometry

RRID：AB_2631357

Storage Buffer：20% ethanol

Storage Condition：Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!