产品信息

     The End-It™ DNA End-Repair Kit is used to convert DNA with damaged or incompatible 5′-protruding and/or 3′-protruding ends  to 5′-phosphorylated, blunt-end DNA for fast and efficient   blunt-end ligation into plasmid, cosmid, fosmid, BAC, other cloning vectors, or nextgen DNA sequencing adaptors. End-repaired DNA can be efficiently and rapidly blunt-end ligated into DNA cloning vectors or to next-gen DNA sequencing adaptors using Lucigen’s Fast-Link™ DNA Ligation Kits.
     End-It DNA末端-修复试剂盒用于将带有受损或不相容的5，-突出和/或3，-突出末端的DNA转化为5，-磷酸化的平末端DNA，用于随后添加下一代DNA测序接头，或用于克隆。用End-It试剂盒处理DNA后，可以使用Fast-Link DNA连接试剂盒（Lucigen）进行快速有效的平末端连接.
优点：
▪ Complete conversion of DNA into blunt-ended, phosphorylated DNA
▪ Repair sheared, nebulized or restriction enzyme-digested genomic DNA
▪ Prepare templates for NGS (next-generation sequencing) or shotgun library preparation
▪ 将DNA完全转化为末端钝化的磷酸化DNA
▪ 修复剪切，雾化或限制性内切酶消化的基因组DNA
▪ 准备用于NGS（二代测序）或shot弹枪文库准备的模板
组成成分：
▪ End-Repair Enzyme Mix：-20°C
▪ End-Repair 10X Buffer：-20°C
▪ ATP：-20°C
▪ dNTPs：-20°C