产品信息

      DNA transformation can be difficult to achieve in many bacterial strains due to the presence of one or more restriction and modification (R-M) systems which cleave unmodified DNA.   TypeOne™ Restriction Inhibitor significantly increases transformation efficiencies of unmodified DNA in bacterial strains with type I R-M systems.1* Developed as a unique preparation of a phage protein called “ocr”,TypeOne Inhibitor can be electroporated into cells along with transforming DNA. In vivo, this protein acts as a molecular decoy that blocks the DNA binding site of type I R-M enzymes and inhibits cleavage of unmodified DNA.
     Type I R-M systems are widespread in Eubacteria and Archaebacteria but are not well characterized.Because TypeOne Inhibitor blocks type I R-M enzymes that recognize different DNA   target sequences its use does not require prior knowledge of the restriction specificity of the host or the restriction sites on the transforming DNA.
     TypeOne Restriction Inhibitor is available in a 100 μg size, at a concentration of 5 μg/μl.
     由于在切割未修饰的DNA时存在一种或多种限制和修饰（R-M）系统，因此在许多细菌菌株中难以实现DNA转化。使用Type I R-M系统，TypeOne™限制性抑制剂可显着提高细菌菌株中未修饰的DNA的转化效率。1*作为独特的噬菌体蛋白“ ocr”制剂开发而成，TypeOne抑制剂可与转化的DNA一起电穿孔进入细胞。 在体内，这种蛋白质充当分子诱饵，可阻断I型R-M酶的DNA结合位点并抑制未修饰DNA的裂解。

     I型R-M系统广泛存在于真细菌和古细菌中，但没有很好的表征。由于TypeOne抑制剂阻断识别不同DNA靶序列的I型R-M酶，因此其使用不需要事先了解宿主的限制性特异性或转化DNA上的限制性位点。

     TypeOne限制性抑制剂的大小为100μg，浓度为5μg/μl。

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参考文献：
1. Hoffman, L.M. et al., (2002) Epicentre Forum 9 (2), 8
2. Walkinshaw, M.D. et al., (2002) Molec. Cell 9, 187.
3. Murray, N.E. et al., (2000) Microbiol. Molec. Biol. Rev. 64,   412.
4. Hoffman, L.M. et al., (2000) Genetica 108, 19.