产品信息

货号	产品名称	规格	价格/元
TNP92110	EZ-Tn5 Transposase/EZ-Tn5转座酶	10 units	6418
EZI982K	EZ-Tn5™ <KAN-2> Insertion Kit/EZ-Tn5™ <KAN-2>插入试剂盒	10 rxns	6950
EZI921T	EZ-Tn5™ <TET-1> Insertion Kit EZ-Tn5™ <TET-1>插入试剂盒	10 rxns	6999
EZI912D	EZ-Tn5™ <DHFR-1> Insertion Kit/EZ-Tn5™ <DHFR-1>插入试剂盒	10 rxns	7060
EZI03T7	EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit/EZ-Tn5™ <T7/KAN-2>启动子插入试剂盒	10 rxns	7312
EZI011RK	EZ-Tn5™ <R6Kγori/KAN-2> Insertion Kit/EZ-Tn5™ <R6Kγori/KAN-2>插入试剂盒	10 rxns	7517
CIS40025	CopyCutter™ Induction Solution/CopyCutter™诱导试剂	25 mL	856

产品简介

      Transposons are mobile DNA sequences found in the genomes of prokaryotes and eukaryotes. Transposon tagging has long been recognized as a powerful research tool for randomly distributing primer binding sites, creating gene“knockouts”, and introducing a physical tag or a genetic tag into large target DNAs. One frequently used transposition system is the Tn5 system isolated from gram-negative bacteria. Though a naturally occurring transposition system,   the Tn5 system can be readily adapted for routine use in research laboratories for the following reasons:
1) Tn5 transposase is a small, single subunit enzyme that has been cloned and purified to high   specific activity.
2) Tn5   transposase carries out transposition without the need for host cell factors.  
3) Tn5   transposon insertions into target DNA are highly random.
4) Tn5 transposition proceeds by a simple“cut and paste”process. Although the chemistry is unique, the result is similar to using a restriction endonuclease, with random sequence specifi city,   accompanied by a DNA ligase activity.
5) Tn5   transposase will transpose any DNA sequence contained between its short  9 basepair Mo saic End (ME) Tn5 transposase recognition sequences.
     In 1998 Goryshin and Reznikoff1demonstrated that a fully functional Tn5 transposition system could be reconstituted in vitro. Additionally, the transposition efficiency of this system has been increased more than 1,000-fold compared to wild-type Tn5 by introducing mutations in the transposase gene and in the 19-bp Tn5 ME transposase recognition sequence.
Lucigen's EZ-Tn5 Transposon Tools (kits and reagents) are based on the hyperactive Tn5 transposition system developed by Goryshin and Reznikoff.
     转座子是在原核生物和真核生物的基因组中发现的可移动DNA序列。 长期以来，转座子标记一直被认为是一种强大的研究工具，可用于随机分布引物结合位点，创建基因“敲除”并将物理标签或遗传标签引入大靶DNA。 一种常用的转座系统是从革兰氏阴性细菌中分离的Tn5系统。 尽管是自然发生的换位系统，但由于以下原因，Tn5系统可以很容易地用于研究实验室的常规使用：
1）Tn5转座酶是一种小的单亚基酶，已被克隆并纯化成高比活性。
2）Tn5转座酶无需宿主细胞因子即可进行转座。
3）Tn5转座子插入目标DNA的随机性很高。
4）Tn5转座通过简单的“剪切和粘贴”过程进行。 尽管化学性质独特，但结果类似于使用限制性核酸内切酶，具有随机序列特异性，并伴有DNA连接酶活性。
5）Tn5转座酶将转置其短9个碱基对的Mo saic End（ME）Tn5转座酶识别序列之间的任何DNA序列。
     1998年，Goryshin和Reznikoff1证明可以在体外重建功能齐全的Tn5转座系统。 此外，通过在转座酶基因和19 bp Tn5 ME转座酶识别序列中引入突变，与野生型Tn5相比，该系统的转座效率已提高了1,000倍以上。
     Lucigen的EZ-Tn5转座子工具（试剂盒和试剂）基于Goryshin和Reznikoff开发的高活性Tn5转座系统。
优点：

        Insert a kanamycin, tetracycline, or DHFR selectable marker into any DNA sequence in vitro
        Skip primer walking - simplify Sanger sequencing of large DNA inserts
        Speed functional analysis without subcloning - create libraries of random mutants from purified DNA
        Minimize insertion bias with the hyperactive Tn5 system, known for highest level of randomness
        将卡那霉素，四环素或DHFR选择标记插入体外任何DNA序列
        跳过引物行走-简化大型DNA插入片段的Sanger测序
        无需亚克隆即可加速功能分析-从纯化的DNA创建随机突变体库
        使用高度活跃的Tn5系统将插入偏差降至最低
组成成分：
               EZ-Tn5™ Transposase 10 U ：储存在-20℃
               EZ-Tn5™ <R6Kγori/KAN-2> Transposon：储存在-20℃
               EZ-Tn5™ 10X Reaction Buffer：储存在-20℃
               EZ-Tn5™ 10X Stop Solution:储存在-20℃
               KAN-2 FP-1 Forward Primer:储存在-20℃
               R6KAN-2 RP-1 Reverse Primer:储存在-20℃
               pUC19/3.4 Control Target DNA:储存在-20℃
               Sterile Water:储存在-20℃
数据：
  

 技术参数
产品优点- Insert a kanamycin, tetracycline, or DHFR selectable marker into any DNA sequence in vitro
- Skip primer walking - simplify Sanger sequencing of large DNA inserts
- Speed functional analysis without subcloning - create libraries of random mutants from purified DNA
- Minimize insertion bias with the hyperactive Tn5 system, known for highest level of randomness
- 将卡那霉素，四环素或DHFR选择标记插入体外任何DNA序列
- 跳过引物行走-简化大型DNA插入片段的Sanger测序
- 无需亚克隆即可加速功能分析-从纯化的DNA创建随机突变体库
- 使用高度活跃的Tn5系统将插入偏差降至最低
产品应用- Introducing a phage T7 RNA polymerase transcriptional promoter into any DNA.
- Faster sequencing of large DNA molecules, as compared to primer walking, random subcloning, or generating nested deletions with exonuclease III and mung bean nuclease.
- Making insertion mutants or gene “knockouts” in vitro.
- Introducing a kanamycin resistance selection marker into any DNA