HighYield T7 Azide RNA Labeling Kit_南京沃博生物科技有限公司

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HighYield T7 Azide RNA Labeling Kit

货号	RNT-101-AZ	售价（元）	3975
规格	25T	CAS号

产品简介
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产品信息

品牌	货号	名称	规格	单价
Jena Bioscience	RNT-101-AZ	HighYield T7 Azide RNA Labeling Kit	25T x 20 μl	3975.00

产品简介

HighYield T7叠氮RNA标记试剂盒旨在通过体外转录产生随机叠氮修饰的RNA探针。这种探针非常适合原位杂交、Northern Blot或交联实验。

使用优化的反应缓冲液和T7 RNA标记聚合酶混合物，5-叠氮-C3-UTP有效地并入RNA中，作为其天然对应物UTP的替代物。35%5-叠氮-C3-UTP取代通常会在反应和标记效率之间实现最佳平衡。然而，5-Azido-C3-UTP/UTP比值的单独优化可以通过单核苷酸格式轻松实现。由此产生的叠氮化物修饰RNA探针随后可通过无铜点击化学用二苯并环辛炔（DBCO）功能化检测试剂（例如DBCO功能化荧光染料或（Desthio）生物素）标记，从而形成稳定的共价标记RNA探针[1]。该试剂盒含有足够的试剂，用于25个标记反应，每个反应20μl（35%5-叠氮-C3-UTP取代，1 mM ATP，GTP，CTP，0.65 mM UTP，0.35 mM 5-叠氮-C3-UTP）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

5-Azido-C3-UTP
1x 20 μl (10 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
DBCO-functionalized detection molecule
RNA purification tools
RNAse-free DNAse I (optional)

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