HighYield T7 Cy5 RNA Labeling Kit

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货号	RNT-101-CY5	售价	4875
规格	20Tx 20 μl	CAS号

产品简介
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产品信息

品牌	货号	名称	规格	单价
Jena Bioscience	RNT-101-CY5	HighYield T7 Cy5 RNA Labeling Kit	20Tx 20 μl	4875.00

产品简介

HighYield T7 Cy5 RNA标记试剂盒旨在通过体外转录产生随机的Cy5修饰RNA探针。这种探针非常适合原位杂交和Northern Blot实验。UTP-X-CY5（也称为GE Healthcare的CY5-17-UTP）通过优化的反应缓冲液和T7 RNA标记聚合酶混合物，作为天然对应物UTP的替代物，有效地并入RNA中。35%UTP-X-Cy5替代通常会在反应和标记效率之间实现最佳平衡。然而，UTP-X-Cy5/UTP比值的个体优化可以通过单核苷酸格式轻松实现。由此产生的Cy5修饰RNA探针随后可通过荧光光谱检测。

该试剂盒含有足够的试剂，用于20个标记反应，每个反应20μl（35%UTP-X-Cy5替代物、2.5 mM ATP、GTP、CTP、0.2 mM UTP、0.1 mM UTP-X-Py5）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

UTP-X-Cy5
1x 10 μl (5 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)

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