肌动蛋白破坏用于研究体外(例如，迁移、胞吞作用)和体内(例如，肿瘤细胞侵袭)的细胞功能。Latrunculin A是一种具有生物活性的2-噻唑烷酮大环内酯，来源于海绵，可隔离G-肌动蛋白并阻止F-肌动蛋白装配。它以1:1的化学计量结合单体肌动蛋白，可用于在体外(Kd = 0.2微米)和细胞内(0.5微米，30分钟)阻断肌动蛋白聚合。[1]、[2]、[3] Latrunculin A (1-10微米)在十分钟内引起肿瘤细胞细胞骨架的解聚。[4]用latrunculin A (10微米)过夜处理细胞强烈抑制肌动蛋白合成。[5]延长的细胞处理阻断了地塞米松诱导的肌动蛋白细胞骨架的变化，而对细胞活力没有影响。[6]

产品性质：

Cas No.：76343-93-6

别名：红海海绵素A,LAT-A

化学名： (R)-4-((1R,4Z,8E,10Z,12S,15R,17R)-17-hydroxy-5,12-dimethyl-3-oxo-2,16-dioxabicyclo[13.3.1]nonadeca-4,8,10-trien-17-yl)thiazolidin-2-one

Canonical SMILES O[C@@](C1)([C@H]2NC(SC2)=O)O[C@H](CC[C@@H](/C=C\C=C\CC/C(C)=C\3)C)C[C@H]1OC3=O

分子式：C22H31NO5S

分子量：421.6

溶解度：25mg/mL in ethanol, or in DMSO

储存条件：Store at -20°C

注意事项：

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References

[1]. Coué, M., Brenner, S.L., Spector, I., et al. Inhibition of actin polymerization by latrunculin A. FEBS Letters 213(2), 316-318 (1987).

[2]. Yarmola, E.G., Somasundaram, T., Boring, T.A., et al. Actin-latrunculin A structure and function. The Journal of Biological Chemisty 275(36), 28120-28127 (2000).

[3]. Loubéry, S., Wilhelm, C., Hurbain, I., et al. Different microtubule motors move early and late endocytic compartments. Traffic 9, 492-509 (2008).

[4]. Hayot, C., Debeir, O., Van Ham, P., et al. Characterization of the activities of actin-affecting drugs on tumor cell migration. Toxicology and Applied Pharmacology 211, 30-40 (2006).

[5]. Lyubimova, A., Bershadsky, A.D., and Ben-Ze'ev, A. Autoregulation of actin synthesis requires the 3'-UTR of actin mRNA and protects cells from actin overproduction. Journal of Cellular Biochemistry 76, 1-12 (1999).

[6]. Liu, X., Wu, Z., Sheibani, N., et al. Low dose latrunculin-A inhibits dexamethasone-induced changes in the actin cytoskeleton and alters extracellular matrix protein expression in cultured human trabecular meshwork cells. Experimental Eye Research 77, 181-188 (2003).